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Posted 12h ago by @br3nna

Tissue culture advice

Okay on a whim I bought three different tissue cultures. Monstera mint, alocasia Polly pink variegated, and platycerium ss foong the staghorn fern.

My plan is to do a 50/50 perlite and fluval stratum mix with a humidity dome for both the alocasia and the monstera but what do you suggest for the staghorn fern?

Is it normal for the agar to appear gray like it does in the image of the staghorn fern? Secondly, how long would you suggest leaving them in the bag of agar after arrival?

Sorry for the book I’m new to tc and have little to no knowledge about platyceriums either loll. Impulse got the best of meπŸ˜‚
#tissueculture #Monstera #Alocasia #Platycerium
@br3nna Hi Brenna! I’ve always had luck putting my tissue cultures directly into their regular substrate after they’ve been β€œdeflasked” and ready to pot up. I use 1/3 coco coir or peat, 1/3 perlite, 1/3 orchid bark, and that s what I would do for the monstera. I am pretty sure I would do that for the alocasia too.

But I’m concerned about the platycerium. That definitely looks like something got into the agar. I would get the platycerium out as soon as you can. For my tc’s after I’ve cleaned all the agar off, I give the roots a quick dunk into a hydrogen peroxide solution. 1 part 3% hydrogen peroxide to 3 parts water for just a minute or two. I’m going to tag Shannon @SuperbRaspfern to be on the safe side.
@DreamMachine Sorry you two. I'll be no help here. I've never worked with tissue cultures.
@DreamMachine thanks the gray agar and brown base on the platycerium is a little worrisome I’m thinking. Worse comes to worse I’ll contact the seller. I think I probably will go ahead and pick up some peat moss and use the mix you suggest since I have two of those substrates already!

Should I use the same mix for the platy or go with the perlite fluval stratum mix? (If it survives lol)
@SuperbRaspfern thank you for checking in even if you didn’t have anything to offer!πŸ₯Ή
@SuperbRaspfern Thank you all the same! @br3nna I think you should contact the seller right away for the platy! Either refund or replacement. But there’s still hope to rehab that one. 🀞

Are you familiar with orchids? Platys are also epiphytes, so they want an extremely airy mix. If you have sphagnum moss I would do a combo of sphag, perlite, and orchid bark. Soak the sphagnum and then wring it out as much as you can and fluff up the strands. People are more likely to have issues with it when it gets too compacted.

If you don’t have sphag, then I would also do the 1/3 peat, 1/3 perlite, 1/3 bark πŸ‘